Manningham Crime Investigation Unit detectives are investigating the theft of money from a gaming machine at Doncaster last month.

Police have been told that two men entered a gaming venue on Williamsons Road around 12.15am on 24 February.

While the men were playing the gaming machines it appears they have noticed a machine that was slightly open.

The men opened the machine and removed the internal cash box that contained an undisclosed amount of money before leaving the venue.

Investigators have released images of two men who they believe may be able to assist with their enquiries.

One of the men is perceived to be Caucasian in appearance, with grey hair and aged in his late 40’s.

He was wearing a grey top and blue jeans.

The other man is perceived to be of Middle Eastern appearance, obese build, between 30 and 40-years-old, with dark hair and beard.

He was wearing a two-tone green coloured top, with dark pants and moccasins.

Detectives are urging anyone with information to contact Crime Stoppers on 1800 333 000 or submit a confidential crime report at www.crimestoppersvic.com.au.